Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro

ABSTRACT Melissa officinalis L., popularly known as lemon balm, is an aromatic plant widely used in medicine, cosmetics, and pharmaceutical industries for its essential oil rich phenylpropanoids, terpenes, phenolics. This study aimed to assess the effect of growth regulators on development physiological biochemical metabolism M. cultured vitro. Seeds were inoculated Murashige Skoog medium added with 6-benzylaminopurine (BAP) 1-naphthaleneacetic acid (NAA) according six different treatments. After 90 days culture, plants evaluated parameters (flavonoids, anthocyanins, chlorophyll). The balance between interfered growth, which increased presence 0.2 mg L-1 BAP. In this treatment, had greater more leaves, biomass production shoots roots was higher than control. Growth did not influence nitrogen assimilation or flavonoid production; however, total chlorophyll anthocyanin indexes enhanced by treatment BAP at concentrations ranging from 0.5 L-1. Auxin improve root but favored callus formation when combined 0.5–3.0 results indicate that high (above 1.0 L-1) should be vitro balm.